16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls

2007/07/12 by J. Michael Janda, Sharon L. Abbott · 10 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Genomics and Phylogenetic Studies #Bacterial Identification and Susceptibility Testing #Mycobacterium research and diagnosis

paper · doi:10.1128/jcm.01228-07

Abstract

The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been by far the most common housekeeping genetic marker used for a number of reasons. These reasons include (i) its presence in almost all bacteria, often existing as a multigene family, or operons; (ii) the function of

Citations

• Intraspecific Variation in Small-Subunit rRNA Sequences in GenBank: Why Single Sequences May Not Adequately Represent Prokaryotic Taxa

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