An improved method for whole protein extraction from yeast Saccharomyces cerevisiae

2011/09/02 by Tingting Zhang, Jie Lei, Hanjiang Yang +3 · 3 citations
Biochemistry, Genetics and Molecular Biology · #Fungal and yeast genetics research #RNA and protein synthesis mechanisms #Microbial Metabolic Engineering and Bioproduction

paper · doi:10.1002/yea.1905

Abstract

A new method for protein extraction from yeast Saccharomyces cerevisiae cells is described. This method involves the use of LiAc and NaOH to enhance the permeability of yeast cell wall prior to protein extraction with SDS-PAGE sample buffer. It was safe and efficient compared to other methods reported so far in the literature. The proteins extracted with this new method retained their immunoreactive properties and are suitable for most applications in molecular biology studies.

Citations

• Cell wall construction in Saccharomyces cerevisiae
• Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
• Rapid and reliable protein extraction from yeast

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