2000/01/01 by Vitaly V. Kushnirov · 6 citations
Biochemistry, Genetics and Molecular Biology · Engineering · Agricultural and Biological Sciences · #Fungal and yeast genetics research #Biofuel production and bioconversion #Fermentation and Sensory Analysis
paper · doi:10.1002/1097-0061(20000630)16:9<857::aid-yea561>3.0.co;2-b
The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates.