Single-cell microfluidic quantification of miRNA-21 and miRNA-34a reveals miRNA interactions in small airway epithelial cells and fibroblasts from patients with COPD

2026/02/13 by Vanessa Ho, Jonathan Baker, Jonathan R. Baker +9
Biochemistry, Genetics and Molecular Biology · Medicine · #Chronic Obstructive Pulmonary Disease (COPD) Research #Extracellular vesicles in disease #MicroRNA in disease regulation

paper · doi:10.1152/ajplung.00292.2025

openalex publication_date 2026/02/13 · openalex created_date 2026/02/14 · openalex updated_date 2026/07/02

Abstract

This study demonstrates a novel microfluidic system that can precisely quantify two specific microRNAs in COPD samples at a single-cell resolution using small quantities of cells and reveal the cellular heterogeneity in cell populations. This dual detection system enables the interrelationship between microRNAs in the same cell to be studied as well as having the ability to monitor microRNA levels during disease progression and therapeutics.

Citations

• Small airway fibroblasts from patients with chronic obstructive pulmonary disease exhibit cellular senescence
• Chronic Obstructive Pulmonary Disease Airway Epithelial Cell–derived Extracellular Vesicles Spread Cellular Senescence via MicroRNA-34a

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