In Vitro Propagation of Rose1

1979/10/01 by Paul M. Hasegawa, Pm Hasegawa · 47 citations
Agricultural and Biological Sciences · Mathematics · #Agriculture and Biological Studies #Flowering Plant Growth and Cultivation #Mathematics #Powdery Mildew Fungal Diseases #Rose (mathematics)

paper · pdf · doi:10.21273/hortsci.14.5.610

published in HortScience 14(5), 610-612 (American Society for Horticultural Science)

openalex publication_date 1979/10/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/05/21

Abstract

Abstract Cultured shoot tips and lateral buds from greenhouse-grown rose ( Rosa hybrida L. cv. Improved Blaze) proliferated multiple shoots on a basal medium (MS salts, vitamins, glycine, sucrose, and agar) supplemented with 3.0 mg/liter 6-benzylamino purine (BA) and 0.3 mg/liter indoleacetic acid (IAA). A 3-fold multiplication was achieved from freshly explanted terminal shoot tips or lateral buds after 8 weeks. Reculture of in vitro- derived shoots onto the same medium resulted in a 6-fold increase in 8 weeks. Roots could be initiated from about 50 % of these shoots after transfer to a medium containing 0.3 mg/liter IAA and 0 or 0.3 mg/liter BA. Regenerated plants were successfully transferred to soil after 2 weeks.

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