1996/08/01 by Chamchuree Sotthikul, Pimchai Apavatjrut
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Agar #Biology #Botany #Callus #Explant culture #Horticulture #In vitro #Kinetin #Meristem #Murashige and Skoog medium #Plant Genetic and Mutation Studies #Plant tissue culture and regeneration #Seed Germination and Physiology #Shoot #Tissue culture
paper · pdf · doi:10.21273/hortsci.31.4.629d
openalex publication_date 1996/08/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/11
Propagation of Curcuma roscoeana in vitro was done successfully by culturing 0.5 × 1.0 mm shoot tips from young buds onto modified MS (1962) + 0.25 mg·L –1 kinetin. The bud-derived new plantlets could be multiplied on a new medium. Stem explants 10 mm in size, measured from base of the plantlets longitudinally cut in half, were the most suitable culture explants providing 2.8 new healthy plantlets/cultured explant. Explants from 4, 6, and 8 weeks old plantlets were more suitable than those of 2 weeks old when grown on agar or in liquid medium. From a histological study, it was found that new buds developed from preexisting meristems. The buds, like root initiation, could also occur directly from initial culture explants, not through callus. The plantlets obtained could successfully be transferred into growing pots, having a 95% survival rate.