1980/01/01 by Tingting Ren, Chengxiao Zhang, Chun-Zhi Jin +10
Biochemistry, Genetics and Molecular Biology · Business, Management and Accounting · Environmental Science · Medicine · #Genomics and Phylogenetic Studies #Global Trade and Competitiveness #Microbial Community Ecology and Physiology #Microbial Natural Products and Biosynthesis
paper · doi:10.1007/s10482-023-01846-9
openalex publication_date 1980/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/23
A polyphasic taxonomic study was conducted on two Gram-negative, non-sporulating, non-motile bacterial strains, S2-20-2<sup>T</sup> and S2-21-1, isolated from a contaminated freshwater sediment in China. Comparative 16S rRNA gene sequence studies revealed a clear affiliation of two strains with Bacteroidetes, which showed the highest pairwise sequence similarities with Hymenobacter duratus BT646<sup>T</sup> (99.3%), Hymenobacter psychrotolerans Tibet-IIU11<sup>T</sup> (99.3%), Hymenobacter kanuolensis T-3<sup>T</sup> (97.6%), Hymenobacter swuensis DY53<sup>T</sup> (96.9%), Hymenobacter tenuis POB6<sup>T</sup> (96.8%), Hymenobacter seoulensis 16F7G<sup>T</sup> (96.7%), and Hymenobacter rigui KCTC 12533<sup>T</sup> (96.5%). The phylogenetic analysis based on 16S rRNA gene sequences showed that two strains formed a clear phylogenetic lineage with the genus Hymenobacter. Major fatty acids were identified as iso-C<sub>15:0</sub>, anteiso-C<sub>15:0</sub>, and summed feature 3 (C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub> ω7c/t) and summed feature 4 (iso-C<sub>17:1</sub> I and/or anteiso-C<sub>17:1</sub> B). Major cellular polar lipids were identified as phosphatidylethanolamine, three unidentified aminolipids, an unidentified aminophosopholipid and an unidentified lipid. The respiratory quinone was detected as MK-7 and the genomic DNA G + C content was determined to be 57.9% (genome) for type strain S2-20-2<sup>T</sup> and 57.7 mol% (HPLC) for strain S2-21-1. The observed ANI and dDDH values between strain S2-20-2<sup>T</sup> and its closely related strains were 75.7-91.4% and 21.2-43.9%, respectively. Based on physiological, biochemical, genetic and genomic characteristics, we propose that strains S2-20-2<sup>T</sup> and S2-21-1 represent a novel species of the genus Hymenobacter, for which the name Hymenobacter sediminicola sp. nov. is proposed. The type strain is S2-20-2<sup>T</sup> (= CGMCC 1.18734<sup>T</sup> = JCM 35801<sup>T</sup>).