Tandem-repeat proteins introduce tuneable properties to engineered biomolecular condensates

2025/01/01 by Tin Long Chris Ng, Mateo P. Hoare, Maria Julia Maristany +10 · 1 voice
Biochemistry, Genetics and Molecular Biology · #RNA Research and Splicing #RNA and protein synthesis mechanisms #RNA modifications and cancer

paper · pdf · doi:10.1039/d5sc00903k

openalex publication_date 2025/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

modelling, demonstrating that the CTPR domain can systematically affect the condensates in a predictable manner. Moreover, we show that incorporating short binding motifs into the CTPR domain results in specific target-protein recruitment into the condensates. Our model system can be rationally designed in a versatile manner to both tune condensate propensity and endow the condensates with new functions.

Citations

• Intrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly
• Valence and patterning of aromatic residues determine the phase behavior of prion-like domains
• Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing
• Highly accurate protein structure prediction with AlphaFold

Discussions

• So excited to finally see our CTPR-condensate work published! Well done Chris, Mateo, Julia et al. ❤️ @chemicalscience.rsc.org @phar.cam.ac.uk @rcollepardo.bsky.social @juliamaristany.bsky.social doi [bsky, 9 points, 0 comments]

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