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MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability

2013/01/16 by Kazutaka Katoh, K. Katoh, D. M. Standley +1 · 1,694 citations
Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #Genomics and Phylogenetic Studies #RNA and protein synthesis mechanisms

paper · pdf · doi:10.1093/molbev/mst010

openalex publication_date 2013/01/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31

Abstract

We report a major update of the MAFFT multiple sequence alignment program. This version has several new features, including options for adding unaligned sequences into an existing alignment, adjustment of direction in nucleotide alignment, constrained alignment and parallel processing, which were implemented after the previous major update. This report shows actual examples to explain how these features work, alone and in combination. Some examples incorrectly aligned by MAFFT are also shown to clarify its limitations. We discuss how to avoid misalignments, and our ongoing efforts to overcome such limitations.

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