2024/01/01 by Georgy Kurakin · 1 voice
paper · doi:10.7490/f1000research.1120000.1
openalex publication_date 2024/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/03/07
In a previously published preprint, we presented an analysis of publicly available data on bacterial lipoxygenases (LOXs), the results of which indicate their possible role in expanding the host range and even affecting representatives of different kingdoms, including plants and humans [1] . The association of bacterial LOXs with affecting cystic fibrosis patients has also been noted. In a literature review, we noted a similar role of LOX and Cif (CFTR inhibitory factor) of Pseudomonas aeruginosa in the colonization of the respiratory tract of cystic fibrosis patients [2] . Based on this, we decided to perform a similar analysis for Cif homologues. We were able to identify a cluster including P. aeruginosa Cif and Acinetobacter nosocomialis Cif on a phylogenetic tree. All proteins in this cluster can be considered as putative Cif's. We performed a systematic review of the ecology of the respective bacterial species carrying these putative Cif's species from the literature in the PubMed database. The obtained ecological profile of bacteria carrying putative Cif's was similar to the ecological profile of lipoxygenase carriers discussed in the preprint [1] . These were also dominated by human/invertebrate/nosocomial bacteria (29%) and plant-associated bacteria (29%). A high percentage of bacteria capable of both infecting humans and colonizing plants were present (17%). More than half (52%) of human-affecting bacteria had been reported as having antimicrobial resistance. This similarity of the ecological characteristics of bacteria might indicate a functional relatedness between Cif's and bacterial LOXs in the host-microbe interaction. Cif had previously been characterized as an epoxide hydrolase that cleaves polyunsaturated fatty acid epoxides and increases the pool of immunosuppressive oxylipins [3] . Given this, it can be assumed that such oxylipin “spoofing” is a universal strategy of bacteria to make cross-kingdom host jumps and affect patients with cystic fibrosis. References [1] G. Kurakin, “Bacterial lipoxygenases are associated with host-microbe interactions and may provide cross-kingdom host jumps”, bioRxiv , 2022.06.21.497025, 2022, doi: 10.1101/2022.06.21.497025 . [2] A.S. Amoah, N.B. Pestov, T.V. Korneenko, I.A. Prokhorenko, G.F. Kurakin, “Lipoxygenases at the Intersection of Infection and Carcinogenesis”, Int. J. Mol. Sci. , vol. 25, p. 3961, 2024, doi: 10.3390/ijms25073961 . [3] K.L. Hvorecny, C.D. Bahl, S. Kitamura, K.S.S. Lee, B.D. Hammock, C. Morisseau, D.R. Madden, “Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase”, Structure , vol. 25, pp. 697-707.e4, 2017, doi: 10.1016/j.str.2017.03.002 .