2012/03/20 by Meng Cui, Ke Si, Cui, Meng +3
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Medical Physics (physics.med-ph) #Optics (physics.optics) #Photoacoustic and Ultrasonic Imaging #Random lasers and scattering media #physics.ins-det #physics.med-ph #physics.optics
paper · pdf · doi:10.48550/arxiv.1203.4400
Three figures
arxiv created 2012/03/20 · openalex publication_date 2012/03/20 · arxiv updated 2012/03/21 · openalex created_date 2019/06/27 · openalex updated_date 2026/07/28
Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in vivo applications, its major limitation is the superficial imaging depth as random scattering in biological tissues causes exponential attenuation of the ballistic component of a light wave. Here we present fluorescence microscopy beyond the ballistic regime by combining single cycle pulsed ultrasound modulation and digital optical phase conjugation. We demonstrate near isotropic 3D localized sound-light interaction with an imaging depth as high as thirteen scattering path lengths. With the exceptionally high optical gain provided by the digital optical phase conjugation system, we can deliver sufficient optical power to a focus inside highly scattering media for not only fluorescence microscopy but also a variety of linear and nonlinear spectroscopy measurements. This technology paves the way for many important applications in both fundamental biology research and clinical studies.