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Single-shot hybrid photoacoustic-fluorescent microendoscopy through a\n multi-mode fiber with wavefront shaping

2020/06/18 by Sylvain Mézil, Mezil, Sylvain, Antonio M. Caravaca-Aguirre +11
Engineering · Physics and Astronomy · #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Optical Coherence Tomography Applications #Optics (physics.optics) #Photoacoustic and Ultrasonic Imaging #Random lasers and scattering media

paper · pdf · doi:10.48550/arxiv.2006.10856

openalex publication_date 2020/06/18 · openalex created_date 2022/07/17 · openalex updated_date 2026/07/28

Abstract

We present a minimally-invasive endoscope based on a multimode fiber that\ncombines photoacoustic and fluorescence sensing. From the measurement of a\ntransmission matrix during a prior calibration step, a focused spot is produced\nand raster-scanned over a sample at the distal tip of the fiber by use of a\nfast spatial light modulator. An ultra-sensitive fiber-optic ultrasound sensor\nfor photoacoustic detection placed next to the fiber is combined with a\nphotodetector to obtain both fluorescence and photoacoustic images with a\ndistal imaging tip no larger than 250um. The high signal-to-noise ratio\nprovided by wavefront shaping based focusing and the ultra-sensitive ultrasound\nsensor enables imaging with a single laser shot per pixel, demonstrating fast\ntwo-dimensional hybrid imaging of red blood cells and fluorescent beads.\n

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