Human microglial cells express a functional IL-12 receptor and produce IL-12 following IL-12 stimulation

2001/11/01 by Yassine Taoufik, Marie-Ghislaine de Goër de Herve, Marie-Ghislaine de Goër de Herve +8 · 10 citations
Immunology and Microbiology · Medicine · Neuroscience · #Biochemistry #Biology #Cell biology #Chemokine receptors and signaling #Colocalization #Cytotoxic T cell #Endosome #Immune Response and Inflammation #In vitro #Interleukin 12 #Intracellular #Molecular biology #Neuroinflammation and Neurodegeneration Mechanisms #Neuroscience #Phosphorylation #Receptor #Stimulation #Tyrosine phosphorylation

paper · pdf · doi:10.1002/1521-4141(200111)31:11<3228::aid-immu3228>3.0.co;2-7

openalex publication_date 2001/11/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

Microglial cells (MC) are IL-12 producers in the central nervous system. Here, we found that IL-12 receptor subunits beta1 and beta2 were both constitutively expressed, and up-regulated by IFN-gamma, in human primary MC. IL-12p70, after binding to its receptor, is internalized into vesicles that qualify as early endosomes as indicated by intracellular colocalization with transferrin. IL-12 induced tyrosine phosphorylation and nuclear translocation of STAT4. IL-12 signaling in human MC also involved members of the NFkappaB family. IL-12p70 and, more effectively, the combination of IL-12p70 and IFN-gamma, induced IL-12p40 mRNA expression and bioactive IL-12p70 production. Human MC, thus, express a functional IL-12 receptor and produce bioactive IL-12 following IL-12 stimulation.

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