Purification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzae

1973/02/01 by Volker M. Vogt · 13 citations
Biochemistry, Genetics and Molecular Biology · #Advanced biosensing and bioanalysis techniques #CRISPR and Genetic Engineering #DNA and Nucleic Acid Chemistry

paper · pdf · doi:10.1111/j.1432-1033.1973.tb02669.x

Abstract

A simple scheme has been devised for the purification of Aspergillus nuclease S 1 from commercially available crude amylase powder. Five purification steps yield 90% pure nuclease with an overall yield of 27%. The enzyme is shown to be absolutely specific for single‐stranded nucleic acids. It degrades both DNA and RNA. The nuclease exhibits a sharp pH optimum at pH 4.2 and is active in low concentrations of dodecylsulfate. Aspergillus nuclease S 1 is a metalloprotein of molecular weight 32000. The utility of this nuclease for measuring annealing of labeled DNA is discussed.

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