2019/10/23 by Kathe, Nina
paper · doi:10.7795/320.201904
The aim was to determine whether small non-coding RNAs (sRNAs) are able to silence antibiotic resistance genes in bacteria. If so, the bacteria would lose their resistance. Therefore, such sRNAs were designed against GFP gene, tetracycline (Tc) and chloramphenicol (Cm) resistance gene. In presence of these sRNAs and the antibiotic, only few Tc resistant bacteria had grown, thus the Tc resistance gene was successfully silenced. In contrast, the number of Cm resistant bacteria remained unchanged and GFP-expressing bacteria continued to glow green, hence those genes were not silenced.