Surfaces and orientations: much to fret about?

2004/12/07 by Ivan Rasnik, Sean A. McKinney, Rasnik, Ivan +4
Biochemistry, Genetics and Molecular Biology · #ATP Synthase and ATPases Research #Advanced Electron Microscopy Techniques and Applications #Biomolecules (q-bio.BM) #FOS: Biological sciences #Mitochondrial Function and Pathology #q-bio.BM

paper · pdf · doi:10.48550/arxiv.q-bio/0412013

9 pages, 7 figures

arxiv created 2004/12/07 · openalex publication_date 2004/12/07 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this review, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.

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