2018/10/30 by Matthias Eibl, Eibl, Matthias, Daniel Weng +13
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Fluorescence Microscopy Techniques #FOS: Physical sciences #Optical Coherence Tomography Applications #Optics (physics.optics) #Spectroscopy Techniques in Biomedical and Chemical Research
paper · pdf · doi:10.48550/arxiv.1810.13018
openalex publication_date 2018/10/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Multi-photon microscopy is a powerful tool in biomolecular research. Less\ncomplex and more cost effective excitation light sources will make this\ntechnique accessible to a broader community. Especially semiconductor diode\nseeded fiber lasers have proven to be robust, low cost and easy to use.\nHowever, their wavelength tuning range is often limited, so only a limited\nnumber of fluorophores can be accessed. Therefore, different approaches have\nbeen proposed to extend the spectral coverage of these lasers. Recently, we\nshowed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS)\ncan be harnessed to red-shift high power pulses from 1064 nm to a narrowband\noutput at 1122 nm and 1186 nm and therefore extend the number of accessible\nfluorophores. In this contribution, we show the applicability of all three\nwavelengths for multi-photon microscopy and analyze the performance.\n