LC/ESI-MS analysis of underivatized amino acids and mass spectrum

2016/08/28 by Yoshinori Takano, Takano, Yoshinori, Yoshito Chikaraishi +3
Biochemistry, Genetics and Molecular Biology · Chemistry · Environmental Science · #Chemical Physics (physics.chem-ph) #FOS: Physical sciences #Isotope Analysis in Ecology #Mass Spectrometry Techniques and Applications #Metabolomics and Mass Spectrometry Studies

paper · pdf · doi:10.48550/arxiv.1608.07780

openalex publication_date 2016/08/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We report the method of LC/ESI-MS analysis of underivatized amino acids with corresponding mass spectrum and fragmentation patterns. Diagnostic product ions determined by mass spectrometry can support the qualitative identification and quantitative estimation of individual amino acids. Therefore, the optimization of chromatographic separation using the ion-pairing reagent (i.e., Nonafluoropentanoic acid, NFPA) is useful for the evaluation of target amino acid and for further compound-specific nitrogen isotope studies of amino acids.

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