Detection and resolution of sub-millimolar concentration amino acids on a benchtop NMR spectrometer by nh-PHIP hyperpolarization

2025/01/01 by Mateusz Urbańczyk, Kärolin Kork, Kerti Ausmees +2 · 1 voice
Chemistry · Materials Science · Biochemistry, Genetics and Molecular Biology · #Advanced NMR Techniques and Applications #Enzyme Structure and Function #Protein Structure and Dynamics

paper · doi:10.1039/d5cc02870a

openalex publication_date 2025/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22

Abstract

Parahydrogen based nh-PHIP hyperpolarization is used to circumvent the sensitivity and resolution limitations of affordable benchtop spectrometers, allowing the detection and resolution of a mixture of dilute amino acids using an 80 MHz instrument.

Citations

• A survey of Hammett substituent constants and resonance and field parameters
• Hyperpolarized 13C NMR Metabolomics of Urine Samples at Natural Abundance Applied to Chronic Kidney Disease

Discussions

• Pleased to share our new paper! We tackle resolution & sensitivity limits in affordable benchtop #NMR using nhPHIP hyperpolarization to resolve a dilute mixture of 8 amino acids—expanding practical be [bsky, 1 points, 0 comments]

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