New Colorimetric Reaction for End-Point, Continuous-Flow, and Kinetic Measurement of Urea

1975/07/01 by David Jung, D.H. Jung, Homer Biggs +4 · 13 citations
Engineering · Chemistry · #Advanced Chemical Sensor Technologies #Analytical Chemistry and Chromatography #Mass Spectrometry Techniques and Applications

paper · doi:10.1093/clinchem/21.8.1136

Abstract

A reaction of urea, o-phthalaldehyde and N-(1-naphthyl)ethylenediamine is described for measurement of urea by manual, continuous-flow, and kinetic methods. The continuous-flow system requires 25 mu-l of sample; 40 samples can be analyzed per hour. The kinetic method requires no enzymes, has no lag phase, and has good sensitivity. A major advantage of the reaction is that it occurs at a temperature of 37 degrees C or lower. The results obtained by all three methods agree well with those for a continuous-flow procedure in which diacetyl is a reagent.

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