A New Simple Semimicro Method for Colorimetric Determination of Urea
1963/02/01 by J J Coulombe, Jean-Franҫois Coulombe, L Favreau +1 · 26 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · #Metabolism and Genetic Disorders #Metabolomics and Mass Spectrometry Studies #thermodynamics and calorimetric analyses
paper · doi:10.1093/clinchem/9.1.102
Abstract
Abstract A new simple and rapid method for quantitative semimicro determination of blood urea is presented. Diacetyl monoxime and thiosemicarbazide form in acid medium with urea a red color complex with a maximum adsorption at 530 mµ stable at room temperature and requiring 0.2 ml. of 1:10 blood filtrate.
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