Rapid Method for the Quantitative Determination of Serum Alkaline Phosphatase

1960/06/01 by Bernard klein, Bernard Klein, Prunella A Read +3 · 3 citations
Chemistry · Medicine · #Alkaline Phosphatase Research Studies #Alkaline phosphatase #Biochemistry #Biology #Chemistry #Chromatography #Enzyme #Erythrocyte Function and Pathophysiology #Organic chemistry #Parathyroid Disorders and Treatments #Phenolphthalein #Phosphate #Phosphate buffered saline #Quantitative analysis (chemistry) #Substrate (aquarium)

paper · doi:10.1093/clinchem/6.3.269

openalex publication_date 1960/06/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/26

Abstract

Abstract A new procedure is described for the simple, rapid, quantitative measurement of serum alkaline phosphatase. The procedure is based on the direct photometric measurement of phenolphthalein released from sodium phenolphthalein phosphate. The substrate is available in a convenient, stabilized form as a tablet containing buffer and substrate in optimum amounts for a single analysis. As many as 30 analyses can be performed in an hour.

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