Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969/09/01 by D W Palmer, David Palmer, Theodore Peters +1 · 2 citations
Medicine · #Potassium and Related Disorders #Renal function and acid-base balance #Antibiotics Pharmacokinetics and Efficacy

paper · doi:10.1093/clinchem/15.9.891

Abstract

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

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