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57Fe enrichment in mice for \beta-thalassaemia studies via Mossbauer spectroscopy of blood samples

2019/03/04 by G. Charitou, Ch. Tsertos, Charitou, G. +9
Physics and Astronomy · #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Medical Physics (physics.med-ph) #Nuclear Experiment (nucl-ex) #nucl-ex #physics.bio-ph #physics.med-ph

paper · pdf · doi:10.48550/arxiv.1903.01245

Preprint, 14 pages including 2 tables and 5 figures; Table 2 has been updated to include new fitting results

arxiv created 2019/04/04 · arxiv updated 2019/04/05

Abstract

In this work, wild-type and heterozygous \beta-thalassaemic mice were enriched with 57Fe through gastrointestinal absorption to characterize in more details the iron complexes appeared in the measured Mossbauer spectra. The 57Fe enrichment method was validated and Mossbauer spectra were obtained at 80K from blood samples from wild-type and \beta-thalassaemic mice at 1, 3, 6, and 9 months of age. As expected, the haemoglobin levels of the thalassaemic mice were lower than from normal mice indicating anaemia. Furthermore, significant amounts of ferritin-like iron were observed in the thalassaemic mice samples, which decreased with mouse age, reflecting the pattern of reticulocyte count reduction reported in the literature.

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