2024/03/13 by Antony C. S. Chan, Chan, Antony C · 1 citation
Computer Science · Engineering · #Advanced Measurement and Detection Methods #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Optical Systems and Laser Technology #Optical measurement and interference techniques
paper · pdf · doi:10.48550/arxiv.2406.15621
openalex publication_date 2024/03/13 · openalex created_date 2024/06/26 · openalex updated_date 2026/07/28
The 96-eyes instrument is capable of computational extended depth of focus (eDOF) of up to +/- 30 micrometer in the phase channel, and conventional depth of field (DOF) of +/- 5 micrometer in the fluorescence channel. However, it requires minimal plate-to-plate cover glass depth variation to function. Plate depths are measured using a third-party plate scanner (Opera Phenix) grouped by plate types (Greiner UV-Star, Cell-Star, and Eppendorf meniscus-free). The two-dimensional (2D) depth dataset is aggregated through principal component analysis to obtain the top eight dominating 2D surface deformation modes. More than 90% of the variation can be explained by the plate's absolute depth and tilt (Pitch, Gradient-Y, and Gradient-X), followed by (~= 2%) the cover glass's curvature (Curve-Y and Curve-XY). Plate-to-plate average depth and tilt variations are suppressed by a customized kinematic mount anchoring the plate's cover glass at the instrument's imaging plane. The plate's average curvature is compensated by manually aligning all 96-eyes microscope objective lenses to track the plate's surface; an one-off calibration procedure aided by the backlash-free piezo-flexure z-stage. Design validation is conducted in silico, with the proof of concept experiment conducted on the 96-eyes with new mounting bracket retrofits.