1998/08/01 by D. W. Piston
Engineering · Biochemistry, Genetics and Molecular Biology · #Photoacoustic and Ultrasonic Imaging #Image Processing Techniques and Applications #Advanced Fluorescence Microscopy Techniques
paper · doi:10.2307/1542768
Microscopic images are characterized by a number of microscope-specific parameters--numerical aperture (NA), magnification (M), and resolution (R)--and by parameters that also depend on the specimen--for example, contrast, signal-to-noise ratio, dynamic range, and integration time. In this article, issues associated with the microscope-specific parameters NA, M, and R are discussed with respect to both widefield and laser scanning confocal microscopies. Although most of the discussion points apply to optical microscopy in general, the main application considered is fluorescence microscopy.