2024/12/13 by Zheng, Kaiyuan, Rush, Katherine W, Date, Swapneeta S +6
#Bacterial Proteins #Cell Membrane #Kinetics #Mercury #Methylation #Methyltransferases #S-Adenosylmethionine #S-adenosyl-L-methionine #Vitamin B 12 #cobalamin #iron-sulfur #methyl mercury #methyltransferase
paper · doi:10.7302/24896
Mercury (Hg) is a heavy metal that exhibits high biological toxicity. Monomethylmercury and dimethylmercury are neurotoxins and a significant environmental concern as they bioaccumulate and biomagnify within the aquatic food web. Microbial Hg methylation involves two proteins, HgcA and HgcB. Here, we show that HgcA and HgcB can be heterologously coexpressed, and the HgcAB complex can be purified. We demonstrated that HgcA is a membrane-associated cobalamin-dependent methyltransferase and HgcB is a ferredoxin-like protein containing two [4Fe-4S] clusters. Further, spectroscopic and kinetic results demonstrate that S-adenosyl-L-methionine (SAM) donates the methyl group to Hg in a two-step reaction involving a methylcob(III)alamin intermediate including Co-thiolate ligation from a conserved Cys residue. Our findings uncover a biological role for SAM in microbial Hg methylation.