2010/12/02 by Yu-Chih Lin, Yu‐Chih Lin, Chau-Jern Cheng +1 · 4 citations
Physics and Astronomy · Engineering · Biochemistry, Genetics and Molecular Biology · #Digital Holography and Microscopy #Optical Coherence Tomography Applications #Advanced Fluorescence Microscopy Techniques
paper · pdf · doi:10.1364/ao.50.000b25
The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3 μm and axial resolution of 10 μm through a thickness of 300 μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.