Single-Atom Ruthenium Biomimetic Enzyme for Simultaneous Electrochemical Detection of Dopamine and Uric Acid

2021/03/10 by Xiaoli Xie, Dong Ping Wang, Chunxian Guo +9 · 215 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · Neuroscience · #Advanced biosensing and bioanalysis techniques #Biochemistry #Catalysis #Chemistry #Combinatorial chemistry #Dopamine #Electrochemical Analysis and Applications #Electrochemical sensors and biosensors #Electrochemistry #Electrode #Enzyme #Neuroscience #Physical chemistry #Ruthenium #Uric acid

paper · doi:10.1021/acs.analchem.0c05191

published in Analytical Chemistry 93(11), 4916-4923 (American Chemical Society)

openalex publication_date 2021/03/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05

Abstract

Single-atom catalysts have attracted numerous attention due to the high utilization of metallic atoms, abundant active sites, and highly catalytic activities. Herein, a single-atom ruthenium biomimetic enzyme (Ru-Ala-C 3 N 4 ) is prepared by dispersing Ru atoms on a carbon nitride support for the simultaneous electrochemical detection of dopamine (DA) and uric acid (UA), which are coexisting important biological molecules involving in many physiological and pathological aspects. The morphology and elemental states of the single-atom Ru catalyst are studied by transmission electron microscopy, energy dispersive X-ray elemental mapping, high-angle annular dark field–scanning transmission electron microscopy, and high-resolution X-ray photoelectron spectroscopy. Results show that Ru atoms atomically disperse throughout the C 3 N 4 support by Ru–N chemical bonds. The electrochemical characterizations indicate that the Ru-Ala-C 3 N 4 biosensor can simultaneously detect the oxidation of DA and UA with a separation of peak potential of 180 mV with high sensitivity and excellent selectivity. The calibration curves for DA and UA range from 0.06 to 490 and 0.5 to 2135 μM with detection limits of 20 and 170 nM, respectively. Moreover, the biosensor was applied to detect DA and UA in real biological serum samples using the standard addition method with satisfactory results.

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