An improved colour reagent for the determination of blood glucose by the oxidase system

1972/01/01 by Denise Barham, P. Trinder · 28 citations
Engineering · Chemical Engineering · Neuroscience · #Electrochemical sensors and biosensors #Analytical Chemistry and Sensors #Photoreceptor and optogenetics research

paper · doi:10.1039/an9729700142

Abstract

A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.

Cited by

• Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
• Effect of dietary N-carbamoylglutamate on milk production and nitrogen utilization in high-yielding dairy cows
• Effects of adrenaline on glucose and glutamine metabolism and superoxide production by rat neutrophils
• Effect of taurine supplementation on hyperhomocysteinemia and markers of oxidative stress in high fructose diet induced insulin resistance. [europepmc]
• The association of higher levels of within-normal-limits liver enzymes and the prevalence of the metabolic syndrome. [europepmc]
• Management of cardiac fibrosis in diabetic rats; the role of peroxisome proliferator activated receptor gamma (PPAR-gamma) and calcium channel blockers (CCBs). [europepmc]
• Antidiabetic and ameliorative potential of Ficus bengalensis bark extract in streptozotocin induced diabetic rats. [europepmc]
• Antidiabetic effect of Withania coagulans in experimental rats. [europepmc]
• Association of blood lactate with carotid atherosclerosis: the Atherosclerosis Risk in Communities (ARIC) Carotid MRI Study. [europepmc]
• Risk assessment and toxic effects of metal pollution in two cultured and wild fish species from highly degraded aquatic habitats. [europepmc]
• Plasma lactate and diabetes risk in 8045 participants of the atherosclerosis risk in communities study. [europepmc]
• The protective effect of fucoidan in rats with streptozotocin-induced diabetic nephropathy. [europepmc]
• Low plasma antioxidant status in patients with epilepsy and the role of antiepileptic drugs on oxidative stress. [europepmc]
• Is there a value for probiotic supplements in gestational diabetes mellitus? A randomized clinical trial. [europepmc]
• Origanum majorana Attenuates Nephrotoxicity of Cisplatin Anticancer Drug through Ameliorating Oxidative Stress. [europepmc]
• The Antidiabetic Activity of Nigella sativa and Propolis on Streptozotocin-Induced Diabetes and Diabetic Nephropathy in Male Rats. [europepmc]
• Vitis vinifera Extract Ameliorate Hepatic and Renal Dysfunction Induced by Dexamethasone in Albino Rats. [europepmc]
• Therapeutic Role of Punica Granatum (Pomegranate) Seed Oil Extract on Bone Turnover and Resorption Induced in Ovariectomized Rats. [europepmc]
• Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system. [europepmc]
• Postharvest biocontrol of Colletotrichum gloeosporioides on mango using the marine bacterium Stenotrophomonas rhizophila and its possible mechanisms of action. [europepmc]
• Ameliorative Effects of Honey, Propolis, Pollen, and Royal Jelly Mixture against Chronic Toxicity of Sumithion Insecticide in White Albino Rats. [europepmc]
• Metabolic effects of exposure to pesticides during gestation in female Wistar rats and their offspring: a risk factor for diabetes? [europepmc]
• Fucoidan from Ascophyllum nodosum Suppresses Postprandial Hyperglycemia by Inhibiting Na + /Glucose Cotransporter 1 Activity. [europepmc]
• Dietary synbiotic supplementation improves the growth performance, body antioxidant pool, serum biochemistry, meat quality, and lipid oxidative stability in broiler chickens. [europepmc]
• Level of Organochlorine Pesticide in Prediabetic and Newly Diagnosed Diabetes Mellitus Patients with Varying Degree of Glucose Intolerance and Insulin Resistance among North Indian Population. [europepmc]
• A Novel PTP1B Inhibitor-Phosphate of Polymannuronic Acid Ameliorates Insulin Resistance by Regulating IRS-1/Akt Signaling. [europepmc]
• Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats. [europepmc]
• Anti-Obesity Effect of Combining White Kidney Bean Extract, Propolis Ethanolic Extract and CrPi 3 on Sprague-Dawley Rats Fed a High-Fat Diet. [europepmc]

Related

• A Colorimetric Method for the Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminases
• Noninvasive Blood Glucose Monitoring
• Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement.
• A more sensitive and stable colorimetric determination of free fatty acids in blood
• A New Simple Semimicro Method for Colorimetric Determination of Urea