2011/03/15 by Guosong Hong, Scott M. Tabakman, Hong, Guosong +13
Chemistry · Engineering · Materials Science · Physics and Astronomy · #Biological Physics (physics.bio-ph) #Carbon Nanotubes in Composites #FOS: Physical sciences #Fullerene Chemistry and Applications #Graphene and Nanomaterials Applications #physics.bio-ph
paper · pdf · doi:10.48550/arxiv.1103.2974
in press at Angewandte Chemie
arxiv created 2011/03/15 · openalex publication_date 2011/03/15 · arxiv updated 2011/03/16 · openalex created_date 2022/10/03 · openalex updated_date 2026/07/28
Low quantum yields of near infrared (NIR) fluorophores have limited their\ncapabilities as imaging probes in a transparent, low background imaging window.\nHere for the first time we reported near-infrared fluorescence enhance (NIR-FE)\ncell imaging using nanostructured Au substrate, which was employed as a general\nplatform for both single-walled carbon nanotubes (SWNTs) and organic\nfluorescent labels in the NIR region. Fluorescence intensity, as well as cell\ntargeting specificity, was greatly improved by this novel imaging technique.\nWith NIR-FE imaging, we were able to image SWNT-stained cells at short exposure\ntime of 300ms, and push the detectable limit of SWNT staining of cells down to\nan ultralow concentration of ~50 pM. Further, different degrees of fluorescence\nenhancement for endocytosed, intracellular SWNTs vs. nanotubes on the cell\nmembrane at the cell/gold interface were observed, suggesting the possibility\nof using this technique to track the transmembrane behavior of NIR\nfluorophores.\n