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Multiplexed Multi-Color Raman Imaging of Live Cells with Isotopically Modified Single Walled Carbon Nanotubes

2008/09/13 by Zhuang Liu, Liu, Zhuang, Xiaolin Li +9
Biochemistry, Genetics and Molecular Biology · Engineering · Neuroscience · Physics and Astronomy · #FOS: Physical sciences #Materials Science (cond-mat.mtrl-sci) #Molecular Communication and Nanonetworks #Photoreceptor and optogenetics research #Spectroscopy Techniques in Biomedical and Chemical Research #cond-mat.mtrl-sci

paper · pdf · doi:10.48550/arxiv.0809.2375

Journal of the American Chemical Society, in press

arxiv created 2008/09/13 · openalex publication_date 2008/09/13 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We show that single walled carbon nanotubes with different isotope compositions exhibit distinct Raman Gband peaks and can be used for multiplexed multi-color Raman imaging of biological systems. Cancer cells with specific receptors are selectively labeled with 3 differently colored SWNTs conjugated with various targeting ligands including Herceptin, anti-Her2, Erbitux, anti-Her1, and RGD peptide, allowing for multi-color Raman imaging of cells in a multiplexed manner. SWNT Raman signals are highly robust against photo-bleaching, allowing long term imaging and tracking. With narrow peak features, SWNT Raman signals are easily differentiated from the auto-fluorescence background. The SWNT Raman excitation and scattering photons are in the near-infrared region, which is the most transparent optical window for biological systems in vitro and in vivo. Thus, SWNTs are novel Raman tags promising for multiplexed biological detection and imaging.

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