2008/10/31 by Zhuo Chen, Scott M. Tabakman, Chen, Zhuo +23
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Biosensing Techniques and Applications #Advanced biosensing and bioanalysis techniques #Biosensors and Analytical Detection #FOS: Physical sciences #Materials Science (cond-mat.mtrl-sci) #cond-mat.mtrl-sci
paper · pdf · doi:10.48550/arxiv.0810.5761
Nature Biotechnology, in press
arxiv created 2008/10/31 · openalex publication_date 2008/10/31 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Detection of biomolecules is important in proteomics and clinical diagnosis and treatment of diseases. Here, we apply functionalized, macromolecular, single walled carbon nanotubes SWNTs as multi-color Raman labels to protein arrays for highly sensitive, multiplexed protein detection. Raman detection utilizes the sharp peaks of SWNTs with minimal background interference, affording a high signal to noise ratio needed for ultra-sensitive detection. Surface-enhanced Raman scattering SERS combined with the strong resonance Raman intensity of SWNTs, affords detection sensitivity down to 1 fM, a three order of magnitude improvement over most of reported fluorescence-based protein detections. We show that human autoantibodies to Proteinase 3 aPR3, a biomarker for the autoimmune disease Wegeners granulomatosis, is detected by Raman in human serum up to a 107 dilution. Moreover, SWNT Raman tags are stable against photobleaching and quenching, and by conjugating different antibodies to pure 12C and 13C SWNT isotopes, we demonstrate two-color SWNT Raman-based protein detection in a multiplexed fashion.