Pixel multiplexing for high-speed multi-resolution fluorescence imaging

2009/10/05 by Gil Bub, Bub, Gil, Matthias Tecza +7
Biochemistry, Genetics and Molecular Biology · #Advanced Fluorescence Microscopy Techniques #Biological Physics (physics.bio-ph) #FOS: Biological sciences #FOS: Physical sciences #Quantitative Methods (q-bio.QM) #Tissues and Organs (q-bio.TO)

paper · pdf · doi:10.48550/arxiv.0910.0789

openalex publication_date 2009/10/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imaging calcium transients in heart cells at 250 Hz with a 10 Hz megapixel camera.

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