2011/06/16 by Jan A.N. Buytaert, Buytaert, Jan A. N., Emilie Descamps +5
Biochemistry, Genetics and Molecular Biology · Engineering · Medicine · #Advanced Fluorescence Microscopy Techniques #FOS: Biological sciences #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Medical Physics (physics.med-ph) #Optical Imaging and Spectroscopy Techniques #Photoacoustic and Ultrasonic Imaging #Tissues and Organs (q-bio.TO)
paper · pdf · doi:10.48550/arxiv.1106.3162
openalex publication_date 2011/06/16 · openalex created_date 2022/10/04 · openalex updated_date 2026/07/28
We report on the recently emerging (Laser) Light Sheet based Fluorescence\nMicroscopy field (LSFM). The techniques used in this field allow to study and\nvisualize biomedical objects non-destructively in high-resolution through\nvirtual optical sectioning with sheets of laser light. Fluorescence originating\nin the cross section of the sheet and sample is recorded orthogonally with a\ncamera. In this paper, the first implementation of LSFM to image biomedical\ntissue in three dimensions - Orthogonal-Plane Fluorescence Optical Sectioning\nmicroscopy (OPFOS) - is discussed. Since then many similar and derived methods\nhave surfaced (SPIM, Ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM...) which we\nall briefly discuss. All these optical sectioning methods create images showing\nhistological detail. We illustrate the applicability of LSFM on several\nspecimen types with application in biomedical and life sciences.\n