2025/07/08 by Ahmed, Naseer
paper · doi:10.48347/imist.prsm/ajmap-v11i1.50603
In nanotechnology, environmentally friendly methods to synthesize metal nanoparticles are becoming increasingly important. This study investigates the potential of plant leaf extracts for the biosynthesis of Au, Fe, and Co nanoparticles. The synthesis and properties of the nanoparticles were confirmed using UV-visible spectroscopy and SEM. The nanoparticles' stability was assessed based on the effects of NaCl, storage, temperature, and pH. Antifungal and antibacterial activities were evaluated using the disc diffusion method. The color of the samples varied at specific ratios for iron, cobalt, and gold nanoparticles, with the optimum absorption spectra peak identified. SEM analysis revealed the average sizes of the nanoparticles, gold 12.6 nm and spherical in shape, cobalt 58.2 nm and shape are amorphous, and iron 25 nm and amorphous. The synthesized nanoparticles demonstrated stability across a range of temperatures (25°C, 50°C, 75°C, and 100°C), as evidenced by no discernible changes in their UV-Vis spectra. While peak broadening was observed in a basic environment, while found stable in an acidic situation. After six months of storage at room temperature and at different concentrations of salt solution, there was no noticeable change in color found, indicating the nanoparticles were stable under various conditions. Au nanoparticles showed an average best activity against all bacterial strains as compare to Co-NPs and Fe-NPs. For antifungal activity iron NPs activity was good against both of the strains as compare to other NPs. This work validates the environmentally friendly production of stable, small-sized nanoparticles from plant leaf extract, which have potential applications in the pharmaceutical sector.