2023/05/14 by Geng Wang, Wang, Geng, Shi, Jindou +3
Biochemistry, Genetics and Molecular Biology · Engineering · Medicine · #Advanced Fluorescence Microscopy Techniques #FOS: Physical sciences #Ocular and Laser Science Research #Optics (physics.optics) #Photonic and Optical Devices
paper · pdf · doi:10.48550/arxiv.2305.08266
openalex publication_date 2023/05/14 · openalex created_date 2023/05/17 · openalex updated_date 2026/08/01
Broad and safe access to ultrafast laser technology has been hindered by the absence of optical fiber-delivered pulses with tunable central wavelength, pulse repetition rate, and pulse width in the picosecond-femtosecond regime. To address this long-standing obstacle, we developed a reliable accessory for femtosecond ytterbium fiber chirped pulse amplifiers, termed as fiber-optic nonlinear wavelength converter (FNWC), as an adaptive optical source for the emergent field of femtosecond biophotonics. This accessory embowers the fixed-wavelength laser to produce fiber delivered ~20 nJ pulses with central wavelength across 950-1150 nm, repetition rate across 1-10 MHz, and pulse width across 40-400 fs, with a long-term stability of >2000 hrs. As a prototypical label-free application in biology and medicine, we demonstrate the utility of FNWC in real-time intravital imaging synergistically integrated with modern machine learning and large-scale fluorescence lifetime imaging microscopy.