Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins

2024/02/04 by Wen, Junpeng, Pilger, Christian, Wang, Wenlong +8
#Biological Physics (physics.bio-ph) #FOS: Physical sciences #Optics (physics.optics)

paper · doi:10.48550/arxiv.2402.02326

Abstract

We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed laser can generate stable pulses at a fundamental repetition rate of 40.71 MHz with a signal-to-noise rate of >100 dB and an integrated relative intensity noise of only ~0.061%. After two-stage external amplification and pulse compression, an output power of ~1.47 W (corresponding to a pulse energy of ~36.1 nJ) and a pulse duration of ~251 fs are obtained. The 1100 nm femtosecond fiber laser is then employed as the excitation light source for multicolor multi-photon fluorescence microscopy of Chinese hamster ovary (CHO) cells stably expressing red fluorescent proteins.

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