γ‐Aminobutyric Acid Release from Neurons and Glia

1976/09/01 by Å. Sellström, Åke Sellström, A. Hamberger +1
Neuroscience · Biochemistry, Genetics and Molecular Biology · #Neuroscience and Neuropharmacology Research #Ion channel regulation and function #Lipid Membrane Structure and Behavior

paper · doi:10.1111/j.1748-1716.1976.tb10307.x

Abstract

Release of 3H-GABA into the medium from bulk-isolated glial cells, neuronal perikarya and synaptosomes has been studied, using a continuous perfusion system. The fractions were incubated with 3H-GABA and spontaneous efflux of label in the presence and absence of aminooxyacetic acid (AOAA) was recorded. Unlabelled GABA at micromolar concentrations markedly stimulated 3H-GABA efflux from glia, synaptosomes and neurons. 3H-GABA from all fractions was also markedly sensitive to stimulation by ouabain. Glutamate was effective in stimulating the spontaneous efflux in the presence of AOAA, while superfusion with a calcium-deprived medium only slightly stimulated the 3H-GABA efflux from neurons and glia.

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