1993/10/01 by Heikki Kainulainen, H. KAINULAINEN, Annette Schürmann +5 · 5 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Amino Acid Enzymes and Metabolism #Biochemistry #Biology #Carbohydrate #Carbohydrate metabolism #Chemistry #Deoxyglucose #Diabetes mellitus #Diet and metabolism studies #Endocrinology #GLUT1 #GLUT3 #GLUT4 #Glucose Transporter Type 1 #Glucose transporter #Glucose uptake #In vivo #Insulin #Internal medicine #Medicine #Metabolism, Diabetes, and Cancer #Streptozotocin #Transporter
paper · doi:10.1111/j.1748-1716.1993.tb09615.x
openalex publication_date 1993/10/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/25
The effects of streptozotocin-induced diabetes (13 weeks) on the in-vivo glucose uptake and on the protein levels of glucose transporters in rat brain were studied and compared with those in cardiac muscle. Diabetes reduced the uptake of 2-[3H]deoxyglucose into lobus frontalis by 70%. However, uptake rates corrected for the 4-fold increase in serum glucose (glucose metabolic index, GMI) were essentially unaltered. The levels of glucose transporter proteins GLUT1 and GLUT3 in crude membranes from brain as assessed by immunoblotting were unaffected by diabetes, whereas GMI and levels of glucose transporters GLUT1 and GLUT4 in heart were reduced by 80 and 65%, respectively. Thus, glucose uptake and levels of glucose transporters in brain, unlike that in insulin sensitive tissues, are normal in long-term hypo-insulinaemia.