2025/05/29 by Gabrielle R. Budziszewski, V. Stojanoff, Sarah Bowman · 2 voices · 3 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · Materials Science · #Artificial intelligence #Biomolecule #Chemical engineering #Chemistry #Computer science #Crystal (programming language) #Crystallization #Crystallography #Diffraction #Engineering #Enzyme Structure and Function #High resolution #Mass Spectrometry Techniques and Applications #Materials science #Nanotechnology #Optics #Physics #Protein Structure and Dynamics #Protein crystallization #Resolution (logic)
paper · pdf · doi:10.1107/s2053230x25004650
published in Acta Crystallographica Section F Structural Biology Communications 81(7), 272-280 (Wiley)
openalex publication_date 2025/05/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Crystal-based structural methods, including X-ray crystallography, are frequently utilized for the determination of high-resolution structures of biomolecules. All crystal-based diffraction methods first require the preparation of biomolecular crystals, and careful sample preparation for crystallization experiments can increase the frequency of success. In this article, strategies to optimize factors that can impact crystallization are presented, from which buffers and reducing agents are most favorable to which crystallization techniques could be used.