2022/10/17 by Vanesa Vinciauskaite, Glenn R. Masson, Glenn R. Masson · 68 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · #Advanced Proteomics Techniques and Applications #Aerospace engineering #Automation #Chemistry #Chromatography #Computer science #Engineering #Heading (navigation) #Hydrogen–deuterium exchange #Mass Spectrometry Techniques and Applications #Mass spectrometry #Materials science #Metabolomics and Mass Spectrometry Studies #Nanotechnology
paper · pdf · doi:10.1042/ebc20220111
published in Essays in Biochemistry 67(2), 301-314 (Portland Press)
openalex publication_date 2022/10/17 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.