Enhancing One Health Through Wastewater-Based Surveillance: Development and Validation of a Multiplex ddPCR Tool to Differentiate Human and Livestock Contributions

2025/11/23 by Andrea Gobbo, Marie‐Alice Fraiture, Marie-Alice Fraiture +14 · 1 voice · 1 citation
Environmental Science · Medicine · #Antibiotic resistance #Digital polymerase chain reaction #Environmental DNA #Fecal contamination and water quality #Harmonization #Human health #Livestock #Multiplex #Pharmaceutical and Antibiotic Environmental Impacts #SARS-CoV-2 detection and testing #Wastewater

paper · doi:10.1016/j.watres.2025.125024

published in Water Research 290, 125024 (Elsevier BV)

openalex created_date 2025/11/23 · openalex publication_date 2025/11/23 · openalex updated_date 2026/07/28

Citations

• 16S rRNA-based assays for quantitative detection of universal, human-, cow-, and dog-specific fecal Bacteroidales: A Bayesian approach
• A multistate assessment of population normalization factors for wastewater-based epidemiology of COVID-19
• Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria

Discussions

• 📢 Nouvelle publication Sciensano dans Water Research ! ➡️ Développement d’un outil ddPCR permettant de distinguer les apports humains et animaux dans les eaux usées. 📄 Publ. : doi.org/10.1016/j.w [bsky, 0 points, 1 comments]