Improved Collagen Extraction by Modified Longin Method
1988/01/01 by T A Brown, Thomas A. Brown, D.E. Nelson +5 · 15 citations
Medicine · Biochemistry, Genetics and Molecular Biology · #Cardiac Valve Diseases and Treatments #RNA and protein synthesis mechanisms #Infective Endocarditis Diagnosis and Management
paper · pdf · doi:10.1017/s0033822200044118
Abstract
A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.
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