Improved Collagen Extraction by Modified Longin Method
1988/01/01 by T A Brown, Thomas A. Brown, D.E. Nelson +5 · 1,078 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Biology #Cardiac Valve Diseases and Treatments #Chemistry #Chromatography #Extraction (chemistry) #Fraction (chemistry) #Infective Endocarditis Diagnosis and Management #Materials science #Medicine #Pathology #RNA and protein synthesis mechanisms #Radiocarbon dating #Reflux #Ultrafiltration (renal)
paper · pdf · doi:10.1017/s0033822200044118
published in Radiocarbon 30(2), 171-177 (Cambridge University Press)
openalex publication_date 1988/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06
Abstract
A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.
Cited by
- Ancient DNA and dating evidence for the dispersal of hippos into central Europe during the last glacial
- On the impact of micro-CT scanning on radiocarbon dating of fossil material: A cautionary note for the palaeoanthropological community and beyond
- AMS Radiocarbon Dating of Ancient Bone Using Ultrafiltration
- What does a bear-baiting assemblage look like? Interdisciplinary analysis of an Early Modern ‘sport’
- Sulfur isotopes evidence spatial ecology of Late Pleistocene ungulates in southwestern France
- Evaluating the Effects of Managed Free-Roaming Cat Populations on Prey Through Stable Isotope Analysis: A Pilot Study from British Columbia, Canada
- The impact of human dispersals and local interactions on the genetic diversity of coastal Papua New Guinea over the past 2,500 years
- Whole-genome ancestry of an Old Kingdom Egyptian
- The earliest unambiguous Neanderthal engravings on cave walls: La Roche-Cotard, Loire Valley, France
- Farming and feasting during the Bronze Age–Iron Age transition in Britain (ca. 900–500 bce): multi-isotope evidence for societal change
- A multiscalar and multiproxy geoarchaeological approach to site formation processes at the Middle and Upper Palaeolithic site of La Roche-à-Pierrot, Saint-Césaire, France
- Chronology, cryostratigraphy, and palaeoenvironmental evidence from the CRREL Permafrost Tunnel, Alaska
- Fatty acid specific δ13C values reveal earliest Mediterranean cheese production 7,200 years ago
- Multidisciplinary study of human remains from the 3rd century mass grave in the Roman city of Mursa, Croatia
- Evaluating ecological relationships and niche breadth among historically sympatric Canis taxa using isotope analysis
- Reconstructing ecological niche and feeding ecology of pre-contact New Zealand avifauna from Harwood, Otago Peninsula
- Increasing Mobility at the Neolithic/Bronze Age Transition - sulphur isotope evidence from Öland, Sweden
- Aurignacian groups at Isturitz (France) adapted to a shifting environment upon their arrival in Western Europe ∼42,000 years ago
- NOthing goes to WAste (NOWA): A protocol to optimise sampling of ancient teeth
- Ancient genomes reveal insights into ritual life at Chichén Itzá
- The Chronological Factor in Understanding the Middle and Upper Paleolithic of Eurasia
- Megafaunal Extinctions and the Disappearance of a Specialized Wolf Ecomorph
- The tale of Henry VII: a multidisciplinary approach to determining the post-mortem practice
- Isotopic Evidence for Maya Patterns of Deer and Dog Use at Preclassic Colha
- Steppe brown bear Ursus arctos “priscus” from the Late Pleistocene of Europe
- Reconstructing Subneolithic and Neolithic diets of the inhabitants of the SE Baltic coast (3100–2500 cal BC) using stable isotope analysis
- History and culture of the early Türkic period: A review of archaeological monuments in the Russian Altai from the 4th–6th century AD
- Ancient DNA extracted from Danish aurochs (Bos primigenius): Genetic diversity and preservation
- Initial Upper Palaeolithic Homo sapiens from Bacho Kiro Cave, Bulgaria
- A 14C chronology for the Middle to Upper Palaeolithic transition at Bacho Kiro Cave, Bulgaria
- The genomic history of southeastern Europe
- Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis
- Coyote ( Canis latrans ) use of marine resources in coastal California: A new behavior relative to their recent ancestors
- Reconstructing Prehistoric African Population Structure
- The genetic prehistory of the New World Arctic
- Tracing the locality of prisoners and workers at the Mausoleum of Qin Shi Huang: First Emperor of China (259-210 BC)
- Radiocarbon evidence for the presence of mice on Madeira Island (North Atlantic) one millennium ago
- Life and extinction of megafauna in the ice-age Arctic
- Ancient genomes reveal structural shifts after the arrival of Steppe-related ancestry in the Italian Peninsula
- Early Pottery at 20,000 Years Ago in Xianrendong Cave, China
- 2000 Years of Parallel Societies in Stone Age Central Europe
- The Quaternary lions of Ukraine and a trend of decreasing size in Panthera spelaea
- On the association of giant short-faced bear (Arctodus simus) and brown bear (Ursus arctos) in late Pleistocene North America
- Ice-age megafauna in Arctic Alaska: extinction, invasion, survival
- Pleistocene megafauna from eastern Beringia: Paleoecological and paleoenvironmental interpretations of stable carbon and nitrogen isotope and radiocarbon records
- Radiocarbon Dates from the Pleistocene Fossil Deposits of Samwel Cave, Shasta County, California, USA
- Giant short-faced bear (Arctodus simus) from late Wisconsinan deposits at Cowichan Head, Vancouver Island, British Columbia
- Implications of Radiocarbon Dates from Potter Creek Cave, Shasta County, California, USA
- Stable isotope dietary analysis of the Tianyuan 1 early modern human
- Sulfur isotopes as a proxy for human diet and mobility from the preclassic through colonial periods in the Eastern Maya lowlands
- A mass sacrifice of children and camelids at the Huanchaquito-Las Llamas site, Moche Valley, Peru
- Diet and Radiocarbon Dating of Tollund Man: New Analyses of an Iron Age Bog Body from Denmark
- Multi-isotopic study of diet and mobility in the northeastern Nile Delta
- The earliest evidence for anatomically modern humans in northwestern Europe
- The First New Zealanders: Patterns of Diet and Mobility Revealed through Isotope Analysis
- Isotope evidence for the intensive use of marine foods by Late Upper Palaeolithic humans
- The Gravettian burial known as the Prince (“Il Principe”): new evidence for his age and diet
- Radiocarbon and Stable Isotope Evidence of Dietary Change from the Mesolithic to the Middle Ages in the Iron Gates: New Results from Lepenski Vir
- First isotope analysis and new radiocarbon dating of Trypillia (Tripolye) farmers from Verteba Cave, Bilche Zolote, Ukraine
- Persistent deathplaces and mobile landmarks: The Holocene mortuary and isotopic record from Wadi Takarkori (SW Libya)
- Wild at heart: Approaching Pitted Ware identity, economy and cosmology through stable isotopes in skeletal material from the Neolithic site Korsnäs in Eastern Central Sweden
- New Radiocarbon Dates and a Review of the Chronology of Prehistoric Populations from the Minusinsk Basin, Southern Siberia, Russia
- The last Neanderthal
- European Middle and Upper Palaeolithic radiocarbon dates are often older than they look: problems with previous dates and some remedies
- The Genomic History Of Southeastern Europe
- IRON AGE DIET AT GLASTONBURY LAKE VILLAGE: THE ISOTOPIC EVIDENCE FOR NEGLIGIBLE AQUATIC RESOURCE CONSUMPTION
- Timing and causes of mid-Holocene mammoth extinction on St. Paul Island, Alaska
- Between the Vinča and Linearbandkeramik Worlds: The Diversity of Practices and Identities in the 54th-53rd Centuries cal BC in Southwest Hungary and Beyond. [europepmc]
- Radiocarbon dating minute amounts of bone (3-60 mg) with ECHoMICADAS. [europepmc]
- A combined method for DNA analysis and radiocarbon dating from a single sample. [europepmc]
- Changing environments during the Middle-Upper Palaeolithic transition in the eastern Cantabrian Region (Spain): direct evidence from stable isotope studies on ungulate bones. [europepmc]
- Pretreatment and gaseous radiocarbon dating of 40-100 mg archaeological bone. [europepmc]
- Genetic turnovers and northern survival during the last glacial maximum in European brown bears. [europepmc]
- Feasting and Mobility in Iron Age Ireland: Multi-isotope analysis reveals the vast catchment of Navan Fort, Ulster. [europepmc]
- The biomolecular characterization of a finger ring contextually dated to the emergence of the Early Neolithic from Syltholm, Denmark. [europepmc]
- New perspectives on Neanderthal dispersal and turnover from Stajnia Cave (Poland). [europepmc]
- Stable isotope evidence for dietary diversification in the pre-Columbian Amazon. [europepmc]
- Proteome Variation with Collagen Yield in Ancient Bone. [europepmc]
- Variable kinship patterns in Neolithic Anatolia revealed by ancient genomes. [europepmc]
- Diversity aboard a Tudor warship: investigating the origins of the Mary Rose crew using multi-isotope analysis. [europepmc]
- Zinc isotopes from archaeological bones provide reliable tropic level information for marine mammals. [europepmc]
- Sulfur isotopes as a proxy for human diet and mobility from the preclassic through colonial periods in the Eastern Maya lowlands. [europepmc]
- Mycobacterium leprae diversity and population dynamics in medieval Europe from novel ancient genomes. [europepmc]
- A 41,500 year-old decorated ivory pendant from Stajnia Cave (Poland). [europepmc]
- Genomic and dietary discontinuities during the Mesolithic and Neolithic in Sicily. [europepmc]
- Trophic position of Otodus megalodon and great white sharks through time revealed by zinc isotopes. [europepmc]
- Optimal linear estimation models predict 1400-2900 years of overlap between Homo sapiens and Neandertals prior to their disappearance from France and northern Spain. [europepmc]
- Proteomics-Compatible Fourier Transform Isotopic Ratio Mass Spectrometry of Polypeptides. [europepmc]
- Stable isotope analyses of amino acids reveal the importance of aquatic resources to Mediterranean coastal hunter-gatherers. [europepmc]
- Parallel worlds and mixed economies: multi-proxy analysis reveals complex subsistence systems at the dawn of early farming in the northeast Baltic. [europepmc]
- Network of large pedigrees reveals social practices of Avar communities. [europepmc]
- Life history and ancestry of the late Upper Palaeolithic infant from Grotta delle Mura, Italy. [europepmc]
Related