2019/01/01 by Marco Grimaldo, Felix Roosen-Runge, Felix Roosen‐Runge +3 · 2 citations
Physics and Astronomy · Biochemistry, Genetics and Molecular Biology · Chemistry · #Spectroscopy and Quantum Chemical Studies #Protein Structure and Dynamics #Mass Spectrometry Techniques and Applications
paper · pdf · doi:10.1017/s0033583519000027
Abstract The dynamics of proteins in solution includes a variety of processes, such as backbone and side-chain fluctuations, interdomain motions, as well as global rotational and translational (i.e. center of mass) diffusion. Since protein dynamics is related to protein function and essential transport processes, a detailed mechanistic understanding and monitoring of protein dynamics in solution is highly desirable. The hierarchical character of protein dynamics requires experimental tools addressing a broad range of time- and length scales. We discuss how different techniques contribute to a comprehensive picture of protein dynamics, and focus in particular on results from neutron spectroscopy. We outline the underlying principles and review available instrumentation as well as related analysis frameworks.