HEAT SHOCK FACTOR A8a Modulates Flavonoid Synthesis and Drought Tolerance

2020/09/21 by Nan Wang, Wenjun Liu, Lei Yu +8 · 210 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · #Abscisic acid #Antioxidant #Biochemistry #Biology #Botany #Cell biology #Chemistry #Drought tolerance #Flavonoid #Gene #Heat shock factor #Heat shock protein #Heat shock proteins research #Hsp70 #Hsp90 #Plant Stress Responses and Tolerance #Plant biochemistry and biosynthesis #Reactive oxygen species #Transcription factor

paper · pdf · doi:10.1104/pp.20.01106

published in PLANT PHYSIOLOGY 184(3), 1273-1290 (Oxford University Press)

openalex publication_date 2020/09/21 · openalex created_date 2020/09/25 · openalex updated_date 2026/08/04

Abstract

). Heat shock factors (HSFs) have well-documented functions in stress responses, but their roles in flavonoid synthesis and the flavonoid-mediated drought response mechanism remain elusive. In this study, we demonstrated that a drought-responsive HSF, designated MdHSFA8a, promotes the accumulation of flavonoids, scavenging of reactive oxygen species, and plant survival under drought conditions. A chaperone, HEAT SHOCK PROTEIN90 (HSP90), interacted with MdHSFA8a to inhibit its binding activity and transcriptional activation. However, under drought stress, the MdHSP90-MdHSFA8a complex dissociated and the released MdHSFA8a further interacted with the APETALA2/ETHYLENE RESPONSIVE FACTOR family transcription factor RELATED TO AP2.12 to activate downstream gene activity. In addition, we demonstrated that MdHSFA8a participates in abscisic acid-induced stomatal closure and promotes the expression of abscisic acid signaling-related genes. Collectively, these findings provide insight into the mechanism by which stress-inducible MdHSFA8a modulates flavonoid synthesis to regulate drought tolerance.

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