Differential Expression of Two Flavonoid 3′-Hydroxylase cDNAs Involved in Biosynthesis of Anthocyanin Pigments and 3-Deoxyanthocyanidin Phytoalexins in Sorghum

2006/08/31 by Chun-Hat Shih, Ivan K. Chu, Wing Kin Yip +1 · 20 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Agronomy #Anthocyanin #Biochemistry #Biology #Botany #Cell biology #Enzyme #Flavonoid #Flavonoid biosynthesis #Gene #Gene expression #Mutant #Photosynthetic Processes and Mechanisms #Phytoalexin #Phytochemicals and Antioxidant Activities #Plant Gene Expression Analysis #Sorghum #Transcriptome

paper · pdf · doi:10.1093/pcp/pcl003

openalex publication_date 2006/08/31 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29

Abstract

Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylated flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.

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