The MYB96 Transcription Factor Regulates Triacylglycerol Accumulation by Activating DGAT1 and PDAT1 Expression in Arabidopsis Seeds

2018/04/04 by Hong Gil Lee, Hyojin Kim, Hyo‐Jin Kim +3 · 27 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · #Acyltransferase #Arabidopsis #Biochemistry #Biology #Biosynthesis #Cell biology #Chemistry #Diacylglycerol kinase #Enzyme #Gene #Lipid metabolism and biosynthesis #Mutant #Plant Molecular Biology Research #Plant responses to water stress #Transcription (linguistics) #Transcription factor #Transcriptional regulation

paper · pdf · doi:10.1093/pcp/pcy073

openalex publication_date 2018/04/04 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06

Abstract

Maturing seeds stimulate fatty acid (FA) biosynthesis and triacylglycerol (TAG) accumulation to ensure carbon and energy reserves. Transcriptional reprogramming is a key regulatory scheme in seed oil accumulation. In particular, TAG assembly is mainly controlled by the transcriptional regulation of two key enzymes, acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) and phospholipid:diacylglycerol acyltransferase 1 (PDAT1), in Arabidopsis seeds. However, the transcriptional regulators of these enzymes are as yet unknown. Here, we report that the R2R3-type MYB96 transcription factor regulates seed oil accumulation by activating the genes encoding DGAT1 and PDAT1, the rate-limiting enzymes of the last step of TAG assembly. Total FA levels are significantly elevated in MYB96-overexpressing transgenic seeds, but reduced in MYB96-deficient mutant seeds. Notably, MYB96 regulation of TAG accumulation is independent of WRINKLED 1 (WRI1)-mediated FA biosynthesis. Taken together, our findings indicate that FA biosynthesis and TAG accumulation are under independent transcriptional control, and MYB96 is mainly responsible for TAG assembly in seeds.

Citations

• MYB94 and MYB96 Additively Activate Cuticular Wax Biosynthesis in Arabidopsis

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