Characterization of Anastomosis Groups of BinucleateRhizoctoniaSpecies Using Restriction Analysis of an Amplified Ribosomal RNA Gene
1991/01/01 by Marc A. Cubeta, M. A. Cubeta · 17 citations
Agricultural and Biological Sciences · Materials Science · Immunology and Microbiology · #Plant Disease Resistance and Genetics #Metallurgy and Material Science #Parasitic Infections and Diagnostics
paper · doi:10.1094/phyto-81-1395
Abstract
Seven U.S. and 16 Japanese binucleate Rhizoctonia anastomosis tester isolates, representing 21 different anastomosis groups, were characterized by restriction analysis of a ribosomal RNA (rRNA) gene. Genomic DNA was extracted from each isolate and a region of DNA coding for a portion of the 25S rRNA (rDNA) was amplified using the polymerase chain reaction. Five tester isolates (CAG1, AGF, AGI, AGJ, and AGK) produced either two or three bands ranging from 1.4 to 1.8 kilobases (kb), whereas five other tester isolates (CAG5, AGBa, AGC, AGD, and AGH) produced a single, 1.8-kb fragment (...)
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